首页  学术报告2014

无标记活细胞层析成像 
发布时间:2017-06-20   浏览次数:

题目:无标记活细胞层析成像

讲座人:周仁杰,香港中文大学电子工程学系助理教授

时间:2017622日(周四)下午1400

地点:光电新大楼1026会议室

报告摘要Quantitative phase microscopy (QPM) precisely maps the amplitude and phase information associated with the electro-magnetic field scattered by objects. Due to its label-free nature, QPM has opened many noninvasive biomedical imaging applications.In this talk, I will first give a brief introduction to QPM and its instrumentation and highlight several important applications. After that, I will focus my topic on tomographic phase microscopy (TPM), which is a 3D imaging technique based on solving the inverse scattering problem with QPM systems. TPM determines 3D refractive index maps of cells with ~ 300 nm lateral resolution and ~ 1 μm axial resolution. Two types of TPM methods will be discussed in details, namely illumination-angle scanning TPM and coherence-gating TPM. For angle-scanning TPM, I will present our recent progress on pushing the 3D imaging speed by implementing digital micromirror devices (DMDs). For coherence-gating TPM, I will first review our earlier work on white-light diffraction tomography (WDT), which used the temporal-coherence gating effect to achieve 1 μm depth resolution. While WDT is based on transmission measurements of forward scattering light, it has the missing cone problem that limited its optical sectioning capability for flat layer samples. This issue can be mitigated by developing a coherence-gating reflection TPM based on using a spatially incoherent dynamic laser speckle illumination. I will share our recent efforts towards accomplishing such a system.

报告人介绍:周仁杰于2014年,2011年,以及2010年分别获得美国伊利诺伊大学香槟分校电子和计算机工程博士学位,美国亚利桑那大学光科学硕士学位,和美国代顿大学电光子硕士学位。周博士现为香港中文大学电子工程学系助理教授和美国麻省理工学院光谱实验室访问助理教授。周博士在光学领域从事研究近十年,在国际重要期刊和会议上以第一作者(或通讯作者)身份发表论文近30余篇,其中包括Nature Photonics Nano Letters Lasers & Photonics ReviewSPIE受邀论文等。周博士过去的研究受到了美国自然科学基金,美国卫生研究员,半导体研究联盟,以及日本滨松公司的资助。此外,他的研究也受到了SPIE Optics and Photonics ScholarshipArnold Beckman Foundation Fellowship的额外资助。周博士还获得了一些研究奖励,例如SPIE Newport Spectra - Physics Research Excellence Travel Grant,伊利诺伊大学P. D Coleman Research Award, Yuen Lo Research Award, Sundaram Seshu Summer Research Award。目前周博士担任Nature Scientific Reports, Optics Letters, Biomedical Optics Express, Optics Express 等近20家国际重要期刊的审稿人。周博士热衷于培养学生,在麻省理工学院,伊利诺伊大学,以及浙江大学指导了10多名本科生和研究生。